cmklr1  (Alomone Labs)

Journal: Nature Genetics

Article Title: Genomic and transcriptomic analyses of aortic stenosis enhance therapeutic target discovery and disease prediction

doi: 10.1038/s41588-025-02417-6

Figure Lengend Snippet: a , Alizarin red staining identifies calcification in human VICs (example with two of eight donors). Silencing of LTBP4 and CMKLR1 significantly suppressed calcification in human VICs cultured in OM. Total n = 11 VIC donors. b , Validation of silencing of LTBP4 and CMKLR1 by siRNA transfection; qPCR. Silencing of LTBP4 and CMKLR1 significantly suppressed their gene expression levels in human VICs (mean ± s.d., n = 8; P < 0.05, two-tailed paired t test). Box plots show the distribution from minimum to maximum values. The box represents the interquartile range (25th to 75th percentiles), with the line inside the box indicating the median. Whiskers extend to the minimum and maximum data points. c , Immunofluorescence staining of LTBP4 and CMKLR1 showed colocalization with calcific nodules and calcifying cells (OsteoSense680; yellow arrows) in human AV ( n = 3 donors). LTBP4 expression in smooth muscle-like cells (orange arrow). CMKLR1 is colocalized with microvessels in human AV (green arrow, inset). Scale bars, 100 µm.

Article Snippet: Gene-specific primers from Life Technologies were used—human GAPDH, Hs02758991_g1; human LTBP4, Hs00943217_m1; human CMKLR1, Hs01081979_s1; human CLCA2, Hs00998923_m1; human CERS2, Hs00371958_g1; human CEP120, Hs00537880_m1.

Techniques: Staining, Cell Culture, Biomarker Discovery, Transfection, Gene Expression, Two Tailed Test, Immunofluorescence, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Molecular dynamics of chemotactic signalling orchestrates dental pulp stem cell fibrosis during aging

doi: 10.3389/fcell.2024.1530644

Figure Lengend Snippet: Chemotactic signals transmitted between fibroblasts and macrophages through Rarres2/Ccrl2/Cmklr1 Axis. (A) UMAP analysis reveals an age-associated increase in Rarres2 expression in fibroblast populations. (B) Cmklr1 expression is consistently observed across all three subsets of myeloid cells during aging. (C) Heatmaps depicting the communication probability between fibroblasts and the other pulp cell populations, mediated by RARRES2 and CMKLR1 interaction. (D) Bar plots showing the mRNA expression level of Ccrl2, Rarres2 and Cmklr1 in mouse incisor pulp from adult and aging groups by qPCR quantification. Sample size, n = 5. ** and *** indicate p < 0.01 and p < 0.001 respectively. (E) Western blot analysis identifies enhanced expression levels of CCRL2, RARRES2, and CMKLR1 in the aged groups. The corresponding quantification of each protein’s expression is shown in the lower panels. Sample size, n = 3. * and *** indicate p < 0.05 and p < 0.001 respectively.

Article Snippet: The membrane was incubated overnight at 4°C with the following primary antibodies: β-actin (20536-1AP,Proteintech, 1:2,500), IL-1β (26048-1-AP, Proteintech, 1:1,000), RARRES2 (10216-1-AP, Proteintech, 1:300), CXCL2 (16325-1-AP, Proteintech, 1; 1,000), CMKLR1 (Bioss,bs-10185R, 1:1,000), CCRL2 (BD-PT0714, Biodragon, 1:1,000).

Techniques: Expressing, Western Blot